Machine study · 10 prompts
Cas9
The 10 review prompts from the notes on Cas9. Answer each in your head, then reveal it. Your grade decides when the card comes back.
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Viruses attack bacteria all the time, and many bacteria fight back with a memory. What does Cas9 do for the bacterium that carries it?
Cas9 is one protein, but it can be aimed at almost any DNA sequence. Where does it carry the address of its target?
Many machines in this atlas burn ATP. What powers Cas9 as it opens DNA and decides to cut?
How it works 2
Read this section: How it works, in the Cas9 notesAt a PAM, the guide pairs with the DNA one letter at a time, like a zipper. What happens when a wrong letter turns up early, next to the PAM?
When all 20 letters of the guide match the DNA, Cas9 brings in its two cutting blades. What does it do to the DNA?
Big picture 4
Read this section: Big picture, in the Cas9 notesWhen a bacterium survives a virus attack, it keeps a record of the invader in its own genome. What does it save?
Cas9 cannot see a matching sequence from afar. What does it check at each spot as it moves along the DNA?
Cas9 checks spot after spot across a whole genome. Roughly how long does one Cas9 take to find its target in a bacterium?
People now use Cas9 to edit genes in patients. Which disease did the first approved Cas9 therapy treat?
Cas9 can stay bound to DNA that only partly matches its guide. So what makes the final call on whether it cuts?
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